Genomics of the Leprosy Bacilli
Summary of Research
The One Health Microbial Evolution Lab develops and applies innovative approaches to sequence leprosy bacilli directly from clinical samples, overcoming the long-standing limitations imposed by the lack of in vitro culture systems. These genomic data are leveraged to investigate the evolution of Mycobacterium leprae across time by integrating modern and ancient DNA, providing a unique perspective on pathogen emergence, diversification, and long-term transmission dynamics. In parallel, we use whole-genome sequencing, molecular profiling, and comparative genomics to identify and monitor mutations associated with resistance to first-line therapies. Candidate resistance markers are functionally assessed and validated using surrogate mycobacterial systems, strengthening the interpretation of genomic findings. Together, these approaches advance our understanding of leprosy biology, support molecular drug-resistance surveillance, and inform the development of improved treatment strategies.